APPENDIX D Nucleotide Sequences, Transcription and Translation Analyses, and Restriction Endonuclease Cleavage Maps of Group-C Human Adenoviruses

Abstract

1. (a) Nucleotide Sequence—Coordinates 0.0–18.0
The sequence of adenovirus-5 DNA from coordinates 0.0–17.0 was determined by Steenbergh et al. (1977); van Ormondt et al. (1978); Maat and van Ormondt (1979); Maat et al. (1980); and C. P. van Beveren, J. Maat, B. M. M. Dekker, J. L. Bos, and H. van Ormondt (all pers. comm.), with additional interpretive contributions by van der Eb et al. (1980) and van Ormondt et al. (1980). The partially overlapping sequence of adenovirus-2 DNA from nucleotides 5858 to 6587 (coordinates 15.9–18.0) was determined by Baker and Ziff (1980), incorporating some data obtained previously by Ziff and Evans (1978) (nucleotides 5996–6246), Akusjärvi and Pettersson (1979b) (nucleotides 5919–6188), and Zain et al. (1979a) (nucleotides 6049–6150). Note that from nucleotide 6247 onward, the sequence shown is that of adenovirus 2. Additional overlapping adenovirus-2 sequences were determined by Arrand and Roberts (1979) (nucleotides 1–157), Shinagawa and Padmanabhan (1979) (nucleotides 1–111), Perricaudet et al. (1979) (nucleotides ~500–650, ~800–1112, and 1229–1632), and Aleström et al. (1980) (nucleotides 3510–4119). When known, differences in adenovirus-2 DNA, relative to adenovirus-5 DNA, are shown above the adenovirus-5 sequence. The following features are marked in the sequence: the boundary of the inverted terminal repetition (ITR) (arrowhead); “TATA” (promoter) sequences (underlined); postulated initiation (ATG) and termination (TAA, TGA, or TAG) codons (boxed); 5′ termini (cap sites) (boxed), AATAAA polyadenylation signals (underlined), and 3′-polyadenylation sites (arrows) of mRNAs; and mRNA splice donor and acceptor sites...