APPENDIX F Nucleotide Sequences, Transcription and Translation Analyses, and Restriction Endonuclease Cleavage Maps of Group-A Human Adenoviruses

Abstract

1. (a) Nucleotide Sequence—Coordinates 0.0–11.2
The DNA sequence of adenovirus 12 (strain Huie) through early regions 1A, 1B, and the protein-IX gene were determined by Sugisaki et al. (1980) (nucleotides 1–2320), by T. Kimura, Y. Sawada, M. Shinagawa, Y. Shimizu, K. Shiroki, H. Shimojo, H. Sugisaki, M. Takanami, Y. Uemizu, and K. Fujinaga (pers. comm.) (nucleotides 1574, 2073, 2319–3860), and by J. L. Bos, L. I. Polder, R. Bernards, P. I. Schrier, P. J. van den Elsen, A. J. van der Eb, and H. van Ormondt (pers. comm.) (nucleotides 1400–3800). This sequence includes nucleotides 6–120 determined by Tolun et al. (1979) and nucleotides 1–200 determined by Shinagawa and Padmanabhan (1980) (but see Appendix H, Fig. H.1 for minor variations). The l-strand DNA sequence (equivalent to r-strand mRNA) is shown. The structures of the RNAs were determined by a combination of nuclease-S1 mapping, cDNA analysis, and homology comparisons with the Group-C viruses (Sawada and Fujinaga 1980; Perricaudet et al. 1980; Bos et al. [see above]). The following features are marked in the sequence: the boundary of the inverted terminal repetition (ITR) (arrowhead); “TATA” (promoter) sequences (underlined); postulated initiation (ATG) and termination (TAG, TGA, TAA) codons (boxed); 5′ termini (cap sites) (boxed), 3′-polyadenylation signals (underlined), and polyadenylation sites (arrowheads) of mRNAs; and mRNA splice sites (brackets). The Roman numerals adjacent to the splice junctions refer to the RNAs in which they occur (see Fig. F.1). Encoded amino acids in their appropriate translation frame are indicated...