APPENDIX F Amino Acid Sequences of Retroviral Structural Proteins

Abstract

All the amino acid sequences presented in this section were derived directly (rather than inferred from nucleic acid sequence; see Appendix E). Protein sequence, when available, is a valuable guide for nucleic acid sequencing; conversely nucleic acid sequence can aid protein sequence determination. The protein and nucleic sequences presented here and in Appendix E are in complete agreement.

1. The Complete Amino Acid Sequence of Ra-MLV and Mo-MLV Pr65^gag
The Mo-MLV Pr65^gag sequence differs from that of Ra-MLV at those positions where the altered amino acid is shown under the Ra-MLV Pr65^gag sequence. The complete structure of Ra-MLV Pr65^gag was determined by sequencing the internal structural proteins p15, p12, p30, and p10, and analyzing a variant of p10 having a four-amino-acid extension at its carboxy terminus. The structural proteins of Mo-MLV have also been completely (p12) or partially (p15, p30, p10) sequenced. Arrows indicate the cleavage sites that have been defined from the amino- and carboxyterminal sequences of the structural proteins (Oroszlan et al., Proc. Natl. Acad. Sci. 75, 1404, 1978) and from the DNA sequence of Mo-MSV (Van Beveren et al., Cell 27, 97, 1981), which is in full agreement with the amino acid sequence. In the p15 region and at a few points in the p30 sequence, the Mo-MSV DNA sequence was used to align the peptides. The DNA sequence for Mo-MLV (Shinnick et al., Nature 293, 543, 1981) is also in full agreement with protein sequence data. The Pr65^gag polyproteins of Ra-MLV and Mo-MLV show approximately 91% identity; the...